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ABSTRACT MUST BE RECEIVED IN BETHESDA BY THURSDAY, 7 FEBRUARY 1974
BIOCHEMISTRY/BIOPHYSICS
Indicate below the numbers and titles of sessions in which your
IMPORTANT:
abstract might be programed (see Topic Category List):
1974
See sample abstracts and typing in-
1 st Choice: #.44. . Title Neuro.chemistry..........
structions on reverse side; use enclosed
2nd Choice #...5.. Title . Protein. .Synthesis
Check-List for preparation of abstract.
3rd Choice: #.2.... Title .Protein Synthesis.
Abstract Form
BIOCHEMISTRY/BIOPHYSICS
POSTER PRESENTATIONS
DIFFERENCES IN IN VITRO PROTEIN SYNTHESIS OF THE CEREBRUM,
As an innovation some contributions will
CEREBELLUM, AND OPTIC LOBES OF CHICK BRAIN, J.W. Yang*, D.S . H.
be scheduled in poster sessions instead of
Liu*, and A. Richardson* (SPON: G. Gorin). Illinois State
as slide presentations. Please indicate
University, Normal, Ill. 61761.
your wishes below:
In vitro protein synthesis by the post-mitochondrial super-
Prefer Indifferent Refuse
natant (PMS ) of the cerebrum (cbr), cerebellum (cbl), and optic
Poster session
lobes (opl) from Leghorn chick brain differ significantly, and
Slide presentation
this difference increases with age. In vitro protein synthesis
by the cor was found to be 2-fold greater than the cbl or opl
while no significant difference was observed between the col or
opl. When the PMS of the three regions of the brain was frac-
tionated by centrifugation into microsomes and post-microsomal
Standard projectors for 2" x 2" a
supernatant (cell sap ), the higher protein synthetic activity
31/4" x 4" slides will be available for slide
of the cor was found to be due to both microsomal and cell sap
presentations. Other audio-visual aids can
factors. Centrifugation of the PMS of the cor, cbl, or opl
be provided at cost if the request and
through sucrose gradients showed that ribosome aggregation to
justification accompany this abstract.
Authors will be billed following the meet-
mRNA was greatest for the cor. The activity of brain ribosomes
ing.
stripped of endogenous mRNA was tested in a system containing
polyuridylic acid (150 pg/ml) and 140-phenylalanine. The in-
corporation of 4C-phenylalanine into acid insoluble material
by the ribosomes from the cor was consistently 10-15% greater
than either the cbl or opl. The three regions of the brain
1. Abstracts must be submitted on this.
also exhibited different sensitivities to aurintricarboxylic
special form for photographic reproduction
acid, an inhibitor of in vitro initiation of protein synthesis.
in the Abstract Volume, together with 7
At a concentration of 50 JM, in vitro protein synthesis by the
photocopies of this face of the form.
PMS from the cor and opl was inhibited 44% and 22% respectively
2. Your abstract should be of the informa-
while no inhibition was observed in the PMS from the col.
tive type, containing: (a) a sentence state-
(Supported by Nutrition Foundation Grant # 471 )
ment of the study's specific objective, unless
this is given by the title; (b) brief statement of methods, if
4. MAILING ADDRESS OF FIRST AUTHOR
pertinent; (c) a summary of the results obtained; (d) a statement of
discussed."
the conclusions. It is NOT satisfactory to state: "The results will be
..Mr . Joe W. Yang
.Biologya Illinois State University
3. Each Abstract Form submitted MUST BE SIGNED by a
.Normal. Illinois.
....... Zip ..61761
member of the American Society of Biological Chemists or the
Biophysical Society.
Telephone No.: Area Code .. 309.. #..436-6289
Mail to:
Member's Signature ...
BIOCHEMISTRY/BIOPHYSICS 1974 MEETING
X ASBC
Biophysical
Attention: Robert A. Harte
9650 Rockville Pike
Telephone No.: Area Code ..4...5......... # 37.2-62
2.GL ex 7250
Bethesda, Maryland 20014

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